Structures

If you want to understand function, study structure
- Francis Crick

Yeast C complex spliceosome

  • Novel intermediate captured after branching
  • Exon-ligation factors (e.g. Prp18) are bound before dissociation of branching factors (e.g. Cwc25) and promote remodelling by the ATPase Prp16
  • C-complex PyMol session

Human P complex spliceosome

  • Human spliceosome captured before mRNA release
  • Some exon-ligation factors (e.g. Prp22, Slu7) are conserved
  • Human-specific exon-ligation factors (e.g. FAM32A) stabilise the active site
  • Human P-complex PyMol Session

 

Yeast P complex spliceosome

  • Stalled before mRNA release
  • Revealed mechanism of 3′-splice site recognition, through pairing with brA and 5′-splice site
  • Yeast P-complex PyMol Session

 

Yeast C* complex spliceosome

  • Stalled right after Prp16 action before stable docking of the 3′-splice site in the active site
  • Revealed exon-ligation conformation of the spliceosome after Prp16 action
  • Exon-ligation factors (Slu7 and Prp18) stabilise the spliceosome after remodelling by Prp16
  • Yeast C* PyMol Session

 

pathway structures